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. 2020 Nov 19;16:448. doi: 10.1186/s12917-020-02671-2

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Inhibition of CDV replication by N-targeted shRNA plasmid vectors. Real-time qRT-PCR (a, c, e) and TCID₅₀ infectivity titration (b, d, f) of the three N-specific plasmid-based shRNA transfections (72 h). Right vertical axis presents the percentage of virus inhibition highlighted on the markers (♦). The error bars represent standard deviations. Values given are the mean ± standard error obtained from three independent experiments. Values followed by the same lower case letters do not differ by Tukey’s test (p < 0.01). BLD, below limit of detection (20 TCID₅₀/mL)