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. Author manuscript; available in PMC: 2020 Nov 19.
Published in final edited form as: Cell Rep. 2020 May 5;31(5):107598. doi: 10.1016/j.celrep.2020.107598

Figure 3. The cAMP Production in Adipocytes Is Required for β-AR-Induced Lipogenic Gene Expression in Swat.

Figure 3.

(A) Gsα-protein inactivation in adipocytes strongly inhibits the β3-mediated stimulation of DNL and induction of browning in sWAT. Depicted are representative western blots for lipogenic (ACLY and FASN) and thermogenic protein (UCP1) expression in sWAT from Gsα-Flox/Flox control and adipocyte-specific GsαKO mice (Adipo-GsαKO) treated with vehicle or with CL316243 for 6 days. GAPDH was used as loading control.

(B) Isolated adipocytes (Adipo) and stromal vascular fractions (SVFs) from Gsα-Flox/Flox or Adipo-GsαKO mice were immunoblotted for Gsα protein.

(C) The effect of adipocyte Gsα inactivation on circulating non-esterified fatty acids (NEFAs) is depicted. *p < 0.05 by Student’s t test.

(D) Quantifications of proteins from the western blots depicted in (A).

(E and F) qRT-PCR analysis to quantify lipogenic (E) and thermogenic (F) gene expressions in sWAT from Gsα-Flox/Flox control and Adipo-GsαKO mice treated or not treated with CL316243.

Graphs show the means ± SEMs. n = 8 mice per group.

(D–F) *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 by 1-way ANOVA, followed by Tukey’s post hoc group comparisons.

See also Figures S1 and S2.