KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-Fatty Acid Synthase, Clone 23 | BD Bioscience | Cat# 610963; RRID:AB_398276 |
| Anti-Fatty Acid Synthase | Abcam | Cat# ab22759; RRID:AB_732316 |
| Anti-UCP1 | Abcam | Cat# ab10983; RRID:AB_2241462 |
| Anti-Tyrosine Hydroxylase | Abcam | Cat# ab75875; RRID:AB_1310786 |
| Anti-Deiodinase-2 | Abcam | Cat# ab77779; RRID:AB_1951738 |
| Anti-ACLY | Proteintech | Cat# 15421-1-AP; RRID:AB_2223741 |
| Anti-ACC | Cell Signaling Technology | Cat# 3662; RRID:AB_2219400 |
| Anti-SCD1 | Cell Signaling Technology | Cat# 2794; RRID:AB_2183099 |
| Anti-pHSL(Ser563) | Cell Signaling Technology | Cat# 4139; RRID:AB_2135495 |
| Anti-phospho PKA substrate (RRXS*/T*) | Cell Signaling Technology | Cat# 9624; RRID:AB_331817 |
| Anti-pCREB(Ser133) | Cell Signaling Technology | Cat# 9198; RRID:AB_2561044 |
| Anti-GAPDH | Cell Signaling Technology | Cat# 2118; RRID:AB_561053 |
| Anti-MCAD | Santa Cruz Biotechnology | Cat# sc-365109; RRID:AB_10709904 |
| Anti-HSP-90 | Santa Cruz Biotechnology | Cat# sc-7947; RRID:AB_2121235 |
| Anti-β-Actin | Sigma-Aldrich | Cat# A2228; RRID:AB_476697 |
| Anti-Rabbit IgG antibody, HRP-conjugated | Millipore | Cat# AP307P; RRID:AB_11212848 |
| Anti-Mouse IgG antibody HRP-conjugated | Millipore | Cat# AP308P, RRID:AB_11215796 |
| Anti-Gsα | Li et al., 2016 | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Tamoxifen | Sigma-Aldrich | Cat# T5648 |
| CL 316,243 | Sigma-Aldrich | Cat# C5976 |
| 6-Hydroxydopamine hydrochloride (6-OHDA) | Sigma-Aldrich | Cat# H4381 |
| Rapamycin | LC Laboratories | Cat# R-5000 |
| Ethanol | American Bio | Cat# AB00515 |
| Polyethylene glycol 300 (PEG 300) | Hampton Research | Cat# HR2–517 |
| Tween 80 | Sigma-Aldrich | Cat# P1754 |
| Tween 20 | Sigma Aldrich | Cat# P1379 |
| 3,3′,5-Triiodo-L-thyronine (T3) | Cayman Chemicals | Cat# 16028 |
| Iodine deficient diet (0.15% PTU) | ENVIGO | Cat# TD.95125 |
| Ingredient matched control diet | ENVIGO | Cat# TD.97350 |
| Bovine serum albumin (fatty acid free) | Sigma-Aldrich | Cat# A8806 |
| D-(+)-Glucose | Sigma-Aldrich | Cat# G8270 |
| Sodium acetate | Sigma-Aldrich | Cat# S5636 |
| Sodium pyruvate | GIBCO | Cat# 11360070 |
| L-Glutamine | GIBCO | Cat# 25030081 |
| [14C]-U-Glucose | Perkin Elmer | Cat# NEC042X250UC |
| DMEM/F-12 | GIBCO | Cat# 11330032 |
| Penicilin/Streptomycin | GIBCO | Cat# 15140122 |
| Normocin | Invivogen | Cat# ant-nr-1 |
| Fetal bovine serum (FBS) | Atlanta Biologicals | Cat# S11550 |
| Phosphate-buffered saline (PBS) | GIBCO | Cat# 14190144 |
| Dibutyryl-cAMP | Tocris Bioscience | Cat# 1141 |
| QIAzol Lysis Reagent | QIAGEN | Cat# 79306 |
| Critical Commercial Assays | ||
| Serum total T3 | Calbiotech, Inc | Cat# T3043T-100 |
| Serum total T4 | Calbiotech, Inc | Cat# T4044T-100 |
| Serum NEFA | Fujifilm Wako Diagnostics | Cat# 999-34691 |
| Free Glycerol Reagent | Sigma-Aldrich | Cat# F6428 |
| Enhanced Chemiluminescent Substrate kit | Perkin Elmer | Cat# NEL104001EA |
| iScript™ cDNA Synthesis Kit | Bio-Rad Laboratories | Cat# 1708891 |
| iTaq Universal SYBR® Green Supermix | Bio-Rad Laboratories | Cat# 1725121 |
| Experimental Models: Cell Lines | ||
| 3T3-L1 cell line | ATCC | Cat# CL-173™; RRID:CVCL_0123 |
| Experimental Models: Organisms/Strains | ||
| Mouse: C57BL/6J | The Jackson Laboratory | Stock# 000664; RRID: IMSR_JAX:000664 |
| Mouse: Gsα-Flox/Flox | Li et al., 2016 | N/A |
| Mouse: Adipoq-cre: B6;FVB-Tg(Adipoq-cre)1Evdr/J | The Jackson Laboratory | Stock# 010803; RRID: IMSR_JAX:010803 |
| Mouse: Adipo-GsαKO | Li et al., 2016 | N/A |
| Mouse: Adipo-Cre: B6.129-Tg(Adipoq-cre/Esr1*)1Evdr/J | The Jackson Laboratory | Stock# JAX:024671, RRID:IMSR_JAX:024671 |
| Mouse: iAdipo-GsαKO, inducible | This paper | N/A |
| Oligonucleotides | ||
| See Table S1 for primers used for qRT-PCR | This paper | N/A |
| Software and Algorithms | ||
| GraphPad Prism 8 | GraphPad | GraphPad Software, Inc |
| Image Lab 5 Analysis Software | Bio-Rad Laboratories | Iten# 1709690 |
| ImageJ | ImageJ | ImageJ.nih.gov |
| Other | ||
| ChemiDoc™ XRS+ Imaging System | Bio-Rad Laboratories | Model# 1708265 |
| Leica DM2500 LED optical microscope | Leica Microsystems | Model# DM2500 LED |
| Leica MC170 HD camera | Leica Microsystems | Model# MC170 HD |