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. Author manuscript; available in PMC: 2020 Nov 19.
Published in final edited form as: Chem Rev. 2020 Jun 19;120(19):11056–11092. doi: 10.1021/acs.chemrev.0c00126

Table 4.

Summary of Applications in Complex Tissues and Organs

Tissue Efforts Toward Optimizing Stem Cell Microenvironment in Bioprinted Constructs References
Adipose
  • Dexamethasone, insulin, methylisobutylxantine, and PPARγ agonist rosiglitazone used to differentiate MSCs

  • Rosiglitazone used independently to differentiate MSCs

  • Decellularized ECM and PCL framework used to differentiate ADSCs

10, 256
10, 257
258, 259
Bone
  • TGF-β superfamily and BMP used to induce differentiation of stem cells toward osteogenic and chondrogenic phenotypes

  • Icroglycerophosphate, insulin, transferrin, selenious acid ,and ascorbate-2-phosphate used to differentiate MSCs to osteogenic phenotype

  • BMP-2 used as bioink additive to induce differentiation of BMSCs

  • MicroRNA sequences miR-23a, miR-30c, MiR-34c, miR-133a, miR-135a, miR-137, miR-204, miR-205, miR-217, miR-218, and miR-338 used to induce osteogenic differentiate through targeting Runx2 transcription factor

  • MicroRNA sequence miRNA-138 used to induce osteogenic differentiation through inhibition of adhesion kinase and signaling cascade

  • HA, and BCPs used to differentiate stem cells in a mouse model

  • PEGDMA supplemented with either HA or bioactive glass used to differentiate MSCs

  • Polylactic acid and polysterene microcarriers used to differentiation MSCs

  • BMP-2 releasing microcarriers used to differentiate goat MSCs

10, 264, 265
268
269
68, 270

68, 271
10, 165
10, 281
10, 189, 192
10, 137
Cartilage
  • BMP-6, EGF, FGF-2, TGF-β1, and other factors used to differentiate ASCs

  • TGF-β1 used to differentiate ASCs

  • TGF-β3, sodium pyruvate, dexamethasone, insulin, transferrin, selenious acid, and ascorbate-2-phosphate used to differentiate MSCs

  • MicroRNA sequence miR-140 used to target IGFBP-5, histone deeylase 4, and Smad3 to cause differentiation

  • MicroRNA sequences miR-199a, miR-18a, miR-221, miR-675, miR-29 and miR-29b used to induce differentiation of stem cells

  • TGF-β1 releasing PLGA microcarriers coated with fibronectin used to differentiate MSCs

  • Nanospheres embedded with TGF-β1 and GelMA supplemented with PEGDA used to differentiate MSCs

  • NR2F2 used to differentiate MSCs

  • Nanofibrillated cellulose with alginate or HA used to differentiate bioprinted human iPSCs

10, 266
10, 267
268
68, 272-274
68, 275-280

193
282
283
284
Cardiovascular & Cardiomyogenic
  • 5-AZA used to differentiation human bone marrow MSCs

  • Scaffolds supplemented with alginate and HA/gelatin used to differentiate cardiomyocyte progenitor cells

  • Thiolate HA hydrogels used to differentiate pre-cardiac cells

  • MircoRNA family miR-15 used to promote viability, resistance, and recovery in setting of ischemic injury

  • HA-based synthetic ECM used to promote proliferation and differentiate stem cells

  • Stem cells bioprinted on decellularized ECM to increase viability, proliferation, and differentiation

  • Embryoid bodies used to differentiate ESCs

  • Microchanneled gelatin hydrogel used to differentiate bioprinted human MSCs

  • 4D bioprinting used to optimize surface pattern technology to differentiate MSCs

10, 286
259, 287, 288

259, 289
68, 290, 291
259, 292
259, 293
259, 294
211
221
Hepatic
  • Media supplemented with activing A, Wnt, and KO-DMEM, followed by bioprinting in alginate hydrogel matrix to differentiate human iPSCs

  • Combination of HGF, basic FGF, BMP-4 and patterned printing in a fibronectin and collagen solution used to differentiate ESCs

  • Replication of hexagonal hepatic subunits and bioprinting on gelatin methacrylate and glycidal methacrylate-HA with HUVECs to differentiate ADSCs

296
259, 297
259, 298
Skeletal Muscle
  • BMP-2 and bioprinting in patterned array on fibronectin-coated glass slides used to differentiation MDSCs

  • ECM-like patterned structure supplemented with FGF-2 and BMP-2 used to differentiation C2C12 cells

  • MicroRNA sequences miR-1, miR-27, miR-133, miR-188, and miR-206 used to differentiate stem cells through transcription factors MyoD and MEF2

  • Topographical alignment of microchannels used to induce MSCs

  • Precise patterned printing to differentiate C2C12 myoblasts

  • Cells printed in alignment instead of random pattern to differentiate C2C12 cells

  • Mimicry of native muscle texture to differentiate bioprinted MDSCs

259, 153
259, 302
68, 303-306
10, 229
259, 281
259, 308
259, 309
Neural
  • Thermoresponsive polyurethane hydrogel bioink used to differentiate NSCs

  • Polysaccharide-based bioink containing alginate, carboxymethyl-chitasan, and agarose used to differentiate printed NSCs

  • Collagen hydrogel supplemented with VEGF used to differentiate mural NSCs

  • Fibronectin-coated PLGA used to differentiate human MSCs

  • FGF-2 and CNTF used to differentiate bioprinted NSCs

  • MicroRNA sequences miR-7, miR-133b, and miR-206 used to promote self-renewal of NSCs, differentiate NSCs and prevent neural degeneration

  • Decreased stiffness in bioprinted matrices used to differentiation MSCs

  • Topographical nanofiber conformations supplemented with retinoic acid loaded PCL nanofibers used to differentiate bioprinted stem cells

  • Bioprinting onto micropatterned PMMA grooved scaffold to differentiate astrocytes

313
314
10, 136
217, 259
316
68, 317-319
312, 320
10, 279
259, 321
Dermal
  • Fibrin-collagen gel used to promote proliferation in bioprinted AFSCs and MSCs

  • Gelatin and fibrin matrix used to differentiate bioprinted MSCs

  • ECM-mimetic created from gelatin and sodium alginate hydrogels to promote regeneration of sweat glands

  • Layered bioprinting and topographical patterning in alginate hydrogel of MSCs, keratinocytes, and fibroblasts to induce microenvironment-mediated tissue formation

36, 259
259, 323
259, 324
5, 259, 325, 326