Fig 1. Experimental design.

Twenty-four hours after seeding the cells, the cell culture medium was changed to a serum-free medium for starvation. The next day, cells were divided into four groups with different conditions (with or without cytokines–static or dynamic). After a further 73 hours, cells were harvested. Two wells of each condition were used for qPCR, Luminex as well as ELISA and one for Western Blot. All samples were taken into account for LDH assay.