Fig 4. IL-2 neutralisation restores Ucp3^-/- T_H17 and iTreg cells to a Ucp3^+/+ phenotype.

Ucp3^+/+ and Ucp3^-/- T_H17 were differentiated in the absence (A) or presence (B) of 10 μg.mL^-1 of anti-IL-2. Relative levels of IL-17A expression by T_H17 cells 72 h post-stimulation were measured by flow cytometry (A, B, with collated data C) and ELISA (G). Levels of FoxP3 expression in iTreg cells generated in the absence (D) or presence (E) of 10 μg.mL^-1 of anti-IL-2 are shown together with collated data (F). Data shown are representative of five independent experiments with similar results. Data were analysed using a student’s t-test to quantify significance where detected (* = p < 0.05; ** = p < 0.01; ** = p < 0.001; N.S. = no significance).