Table 1.
Experimental groups and mortality rate.
| Experimental groups | Neurological test Brain water content |
ELISA | IF staining | WB | TUNEL | Exclusion | Mortality | Subtotal |
|---|---|---|---|---|---|---|---|---|
| Experiment 1 | ||||||||
| Sham | 4 | 6 | 0 | 0 | 10 | |||
| ICH (6 h, 12 h, 24 h, 72 h, and 7 d) | 4 | 6 × 5 | 3 | 6 (13.95%) | 43 | |||
| Experiment 2 | ||||||||
| Sham | 6 | 6 | 0 | 0 | 12 | |||
| ICH + PBS | 6 | 6 | 0 | 2 (14.29%) | 14 | |||
| ICH + NDP-MSH (1.5 μg/mouse) | 6 | 6 | 0 | 2 (14.29%) | 14 | |||
| ICH + NDP-MSH (5 μg/mouse) | 6 | 6 | 0 | 0 | 12 | |||
| ICH + NDP-MSH (15 μg/mouse) | 6 | 6 | 2 | 2 (12.5%) | 16 | |||
| Experiment 3 | ||||||||
| Sham | 4 | 0 | 0 | 4 | ||||
| ICH + PBS | 6 | 4 | 0 | 0 | 10 | |||
| ICH + NDP-MSH | 6 | 4 | 0 | 1 (9.09%) | 11 | |||
| ICH + NDP-MSH + Scr siRNA | 6 | 6 | 0 | 0 | 12 | |||
| ICH + NDP-MSH + Mc1r siRNA | 6 | 6 | 4 | 1 | 4 (19.05%) | 21 | ||
| Experiment 4 | ||||||||
| ICH + NDP-MSH + DMSO | 6 | 6 | 0 | 1 (7.69%) | 13 | |||
| ICH + NDP-MSH + LY294002 | 6 | 6 | 4 | 1 | 2 (10.53%) | 19 | ||
| Total | 30 | 54 | 8 | 72 | 20 | 7 | 20 (9.48%) | 211 |
ICH: intracerebral hemorrhage; PBS: phosphate-buffered saline; Mc1r: melanocortin-1 receptor; LY294002: PI3K specific inhibitor; siRNA: small interfering RNA.