FIG 2.

Bar plots showing the taxonomic classification accuracy of each primer pair under two classification schemes. DADA2 taxonomic annotation gives higher taxonomic resolution than mapping to a comparable database, both in general and for Lactobacillus in particular. The entire OptiVag database was extracted in silico with each of the candidate primer sets, without errors. (Top left) The complete database, annotated by mapping; (top right) the complete database, annotated with DADA2’s algorithm; (bottom left) same as panel a but focusing only on Lacobacillus; (bottom right) same as panel b but focusing only on Lactobacillus.