FIG 5.

Effect of human-DNA removal strategies on the amount of bacterial and human DNA retained. The human-DNA content in the 8 pools varied from 86 to 98% of the total DNA content. Different DNA removal methods have various amounts of human DNA left in the filtered sample but also retain various amounts of the original microbial pool. Kraken and BMTagger remove most human DNA but also the most microbial reads. (a) Percentage of human reads in each sample before and after each human removal strategy. (b) Percentage of the original pool of microbial reads kept in each sample after each human removal strategy. (c) The two measurements in a and b are combined into a scatterplot to give an overview of the performance of each tool.