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. 2020 Jun 7;69(4):430–440. doi: 10.1538/expanim.20-0046

Table 2. Primers used in this study.

Gene Forward primer (5’→3’) Reverse primer(5’→3’)
KLF5 F: CGCGCTCGGATGAATTAACG R: GATACAGAACAGCCTCGGCA
Atrogin 1 F: TCAACGGGTCGGCAAGTCT R: TCCCTCCCATCGCTCAGTC
MuRF1 F: GGCAGCAGCATCATCTCGG R: CCTCGCAGGTGACGCAGTAG
β-actin F: GTCCACCGCAAATGCTTCTAA R: TGCGCATTTATGGGTTTTGTT
LEF1 F: CATCAAGTCCTCGCTGGTCAACG R: TAGGAATCCTGCGCTGGAGTCTG
CYP1A1 F: ACGGACTCATTGATTGGGCA R: GGGTACAAGGCAGCGTACAT
MYOM1 F: GCAGTCAACCGTAAGCCAGTACC R: CTGTAGGCTGCTGACTCAAGGTTG
NOB1 F: AGGTGTTGGCGCTCACG R: GCATCTCCTGGAGCTCATCC
MYF6 F: AGCGGAGGAGGCTGAAGAAGATC R: TGATGGCGCTCCTCAGGATCTC
Wnt3a F: GTGGCTTTTGCAGTGACCAG R: GTTGTGCCTGTTCATGGCTG
Wnt5a F: TGGCTTCTCAGTACCTCGTAGTGG R: TAGAGACCACCAAGAGCTGGCTTC
Wnt5b F: GTGCCATCAAGGAGTGCCAGTAC R: GCCGAAGACGGACGTGTTGTC
Wnt7a F: CTCCGCCGACATCAGATACG R: CCCGGAATTTCGGCAGAGTT
Wnt10a F: GCCAACACGGTGTGCCTGAC R: CGTGGATGGCGATCTGGATGC
β-catenin F: TACTGCAGGCACACTACACA R: TTTGTCTTGTTGAGCAGGGC