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. 2020 Jul 16;69(4):448–460. doi: 10.1538/expanim.19-0153

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©2020 Japanese Association for Laboratory Animal Science

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.

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Fig. 3. — Effect of EA on neuronal apoptosis in the substantia nigra of 6-OHDA-induced rats. A. TH positive cells in the substantia nigra of rats from NC group, Sham group, 6-OHDA group, and 6-OHDA+EA group were stained after 6-OHDA lesioning. Scale bar=200 µm. B. Photomicrographs showed the TUNEL positive cells (green) in the substantia nigra of rats from each group. DAPI (2-4-Amidinophenyl-6-indolecarbamidine dihydrochloride) was used to stain nuclei (blue) for light microscopy. Scale bar=100 µm. C. Western blot showed the expression of cleaved caspase-3 and cleaved PARP in the substantia nigra of rats from each group. Data are expressed as mean ± SD. **P<0.01.