Fig. 2.

The survival rate of vitrified-warmed embryos after cold storage. The embryos were stored for 0, 24, 48, 72, or 96 h before vitrification. The survival rate was calculated as follows: (Number of morphologically normal embryos/Number of collected embryos) × 100. Results are expressed as the mean ± SD (n=4–5). More than 39 embryos were analyzed in each experiment, and the total number of analyzed embryos in each experimental group was as follows (0 h: 227, 24 h: 170, 48 h: 172, 72 h: 226, and 96 h: 227).