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. 2020 Jun 17;69(4):423–429. doi: 10.1538/expanim.20-0042

Fig. 3.

Fig. 3.

The developmental ability of vitrified-warmed embryos after cold storage. The embryos stored in the refrigerator for 0, 24, 48, 72, or 96 h (A: 0 h, B: 24 h, C: 48 h, D: 72 h, E: 96 h) and then vitrified. The developmental rate was calculated as follows: (Number of four-cell embryos, morulae, or blastocysts/Number of two-cell embryos) × 100. Results are expressed as the mean ± SD (n=4–15). *P<0.05 compared with Cold-stored embryos. More than 27 embryos were analyzed in each experiment, and the total number of analyzed embryos in each experimental group was as follows (0 h-Cold: 457, 0 h-Vitrified-Cold: 328, 0h-Cold-Vitrified: 197, 24 h-Cold: 397, 24 h-Vitrified-Cold: 236, 24 h: Cold-Vitrified: 166, 48 h-Cold: 426, 48 h-Vitrified-Cold: 324, 48 h-Cold-Vitrified: 167, 72 h-Cold: 397, 72 h-Vitrified-Cold: 257, 72 h-Cold-Vitrified: 205, 96 h-Cold: 456, 96 h-Vitrified-Cold: 227, and 96 h-Cold-Vitrified: 208).