Table 2.
Results of the TFEC Reconstruction for Holo-ACBP
| Δxc (nm) | fc (pN) | ΔG0/(kBT) | |
|---|---|---|---|
| Data Set 1 (118 cycles) | 11.4 ± 0.6 | 7.5 ± 0.5 | 14.3 ± 1.7 |
| Data Set 2 (131 cycles) | 11.4 ± 0.8 | 7.7 ± 0.6 | 14.4 ± 2.3 |
| Data Set 3 (150 cycles) | 11.1 ± 1.0 | 7.0 ± 0.8 | 13.3 ± 3.5 |
| Data Set 4 (159 cycles) | 11.0 ± 0.9 | 7.6 ± 0.8 | 13.8 ± 3.9 |
| Data Set 5 (109 cycles) | 10.9 ± 1.2 | 6.5 ± 0.8 | 12.5 ± 4.9 |
| Weighted Average | 11.2 ± 0.4 | 7.4 ± 0.3 | 14.1 ± 1.2 |
Here, ΔG0 is the zero-force free energy difference between the fully denatured apo-ACBP and the native holo-ACBP states. Each data set was acquired from one molecule.