Table 3.
Results of the TFEC Reconstruction for Apo-ACBP
| Δxc (nm) | fc (pN) | ΔG0/(kBT) | |
|---|---|---|---|
| Data Set 1 (199 cycles) | 8.6 ± 0.4 | 5.8 ± 0.3 | 7.7 ± 0.8 |
| Data Set 2 (284 cycles) | 8.0 ± 0.3 | 5.7 ± 0.3 | 6.6 ± 0.5 |
| Data Set 3 (286 cycles) | 7.9 ± 0.3 | 6.0 ± 0.3 | 6.5 ± 1.4 |
| Data Set 4 (100 cycles) | 7.6 ± 0.7 | 5.2 ± 0.6 | 6.5 ± 1.8 |
| Weighted Average | 8.1 ± 0.2 | 5.8 ± 0.2 | 6.9 ± 0.4 |
Here, ΔG0 is the zero-force free energy difference between the fully denatured apo-ACBP and the native apo-ACBP states. Each data set was acquired from one molecule.