Table 2.

Association and Dissociation Rate Constants Calculated from ABF Simulations for AMT⁺ or RMT⁺ in WT or M2 S31N

ABF Rate Constant Results
	k1 (M−1 s−1) = kc × e−ΔG/kT	k2 (s−1)	EP k1 (M−1 s−1)	EP k2 (s−1)
WT M2(+1) AMT+	380.9	1.11 × 10−4	372	2.3 × 10−4
WT M2(+1) RMT+	617.6	1.27 × 10−4	659	1.58 × 10−4
S31N M2(+1) RMT+	1.61 × 105	24.9	3622	2.73

Electrophysiologically measured rate constants (EP) are included for reference.