Skip to main content
. 2020 Sep 28;119(9):1833–1848. doi: 10.1016/j.bpj.2020.09.019

Figure 3.

Figure 3

Orthosteric and allosteric changes upon PIFtide binding. (A) Deuterium exchange difference plot (deuterons versus peptic cleavage peptides displayed from the N- to C-terminus) comparing differences in deuterium exchange between PIFtide-bound and apo PDK1 at different deuterium exchange time points, as indicated in key. Axes, time points, error curves, and highlighted regions of interest are similar to Fig. 2A. (B–D) Semilog deuterium uptake plots showing deuterons exchanged with time (minutes) for selected peptides spanning residues lining the PIF-pocket for apo (red) and PIFtide-bound (green) PDK1. (E–G) Deuterium exchange differences between PIFtide-bound and apo-PDK1 at t = 1, 10, and 100 min, respectively, mapped to structure of PDK1-ATP-PS48 complex (PDB: 3HRF). Regions showing decreased exchange are indicated in shades of blue, whereas those showing increased exchange (positive differences) in shades of red, as per key. See also Fig. S2; Tables S1, S3, and S4. To see this figure in color, go online.