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. 2020 Nov 6;11:576736. doi: 10.3389/fphys.2020.576736

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Copyright © 2020 Lasch, Kumaraswami, Nasiscionyte, Kircher, van den Heuvel, Meister, Ishikawa-Ankerhold and Deindl.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effect of bovine RNase A on macrophage recruitment and polarization. The scatter plots show the ratio (ischemic vs. non-ischemic tissue) of (A) total CD68⁺ cells and the percentage of (B) CD68⁺/MRC1⁻ and of (C) CD68⁺/MRC1⁺ cells of all CD68⁺ cells in the gastrocnemius muscle of RNase A or saline treated mice 7 days after the surgical procedure. Data are means ± S.E.M., n = 3 per group, 10 cross-sectional areas (450 μm × 450 μm each) of the gastrocnemius muscle were analyzed per mouse per leg. ^*p < 0.05 (RNase A vs. saline treated group) by unpaired student’s t-test. (D) Representative immunofluorescence pictures of analyzed gastrocnemius tissue of saline‐ (upper picture) or RNase A (lower picture) treated mice 7 days after FAL. Scale bars 100 μm.