Extended Data Fig. 6. NGF and IGF-1 drive RGC axon regeneration in a collaborative manner.

a, Quantification of a panel growth factors in unconditioned media (black bars) and NCM (gray bars) by multiplexed antibody array. b, Primary RGC were cultured in the absence or presence of recombinant mouse CNTF, IGF-1, NGF, or a combination of IGF-1 and NGF. Neurite length was measured 24 hours later. Each symbol represents the mean of 200 RGCs in one independent experiment (n=6 independent experiments shown). Statistical significance was determined by one-way ANOVA followed by Tukey’s post hoc test (**P < 0.01, ***P < 0.001 compared with No Tx; ^#P < 0.05 compared with NGF; ⁺⁺P < 0.01, compared with IGF-1). c, Recombinant IGF-1 (blue bars), NGF (green), a combination of NGF and IGF1 (white), or PBS alone (black) was injected into the vitreous on days 0 and 3 post ONC injury. Optic nerves were harvested 14 days later. Density of regenerating axons in optic nerve sections, at serial distances from the crush site (n= 8 nerves per group). One experiment representative of 2 with similar results is shown. Statistical significance was determined by one-way ANOVA followed by Tukey’s post hoc test (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 compared with PBS; ^#P < 0.05 compared with NGF; ⁺P < 0.05, ⁺⁺P < 0.01, compared with IGF-1). b,c, Data shown as mean +/− sem.