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. Author manuscript; available in PMC: 2021 Apr 26.
Published in final edited form as: Nat Immunol. 2020 Oct 26;21(12):1496–1505. doi: 10.1038/s41590-020-00813-0

Extended Data Fig. 2. Immature neutrophils are mobilized into the circulation following treatment with i.o. zymosan and i.p. αCXCR2.

Extended Data Fig. 2

Mice received an i.o. injection of zymosan on day 0, and i.p. injections of NRS (blue) or αCXCR2 (red) on days 0 , 2 and 4, post ONC injury. Peripheral blood cells were obtained on day 5 and analyzed by flow cytometry. a, Cell surface expression of Ly6G, CD14 and CD101. Upper panels, representative histograms. Lower panels, geometric Mean Fluorescence Intensity on gated Ly6G+ cells and percentage of CD101+ neutrophils. Each symbol represents data from an individual mouse (n=3 mice/ group). Data are shown as mean +/− sem. One experiment representative of 3 with similar results is shown. Statistical significance was determined by two tailed unpaired Student’s t-test. b, Representative dot plots.