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. Author manuscript; available in PMC: 2021 Apr 7.
Published in final edited form as: Nature. 2020 Oct 7;587(7834):426–431. doi: 10.1038/s41586-020-2807-6

Figure 4. Auditory cortex and the oxytocinergic system are required for the re-tuning of cortical neurons during cohousing.

Figure 4.

a, Playback of slow morphs (ISI:575 ms) during cohousing (CH+575; step 1=cohousing). b, Excitatory tuning in CH+575 (N=6) and CH (cohoused) virgins (N=5) (575, p=0.007; one-way ANOVA, Bonferroni correction). Mean±s.e.m. c, Retrieval in cold pup assay. CH (N=9–17 mice, n=14–26 trials), CH+575 (N=8–9, n=12–16); slow, p=0.001. d, Left, ACtx photoinhibition during morph playback on schedule in (a). Right, retrieval in cold pup assay. CH+575sham (N=3 mice, n=12 trials each), CH+575opto-ACtx (N=4, n=16); slow, p=0.02. e, Left, photoinhibition of oxytocin neurons on schedule in (a,d). Right, retrieval in cold pup assay. CH+575sham (N=3 mice, n=12–13 trials), CH+575opto-OT (N=5, n=16–19); warm, p=0.008; prototypes, p=0.02; slow, p=0.01. f, Temporal tuning in CH+575opto-OT virgins (N=4 mice; pan-neuronal GCaMP6f). Mean±s.e.m. g, Tuning curve widths (75:375 ms). CH+575opto-OT virgins from (f) vs. CHexc (N=14 mice, p=0.008) and CHinh (N=10, p=0.03). Median±interquartile. h, Cumulative distribution of tuning widths from CH+575opto-OT virgins (N=4 mice, n=40 tuning curves) vs. virgins retrieving on 100% (CH100%, N=2, n=52; p<0.0001) or 10–30% of trials (CH10–30%, N=2, n=78; p>0.99). Statistics: c,d,e, two-tailed Fisher’s test, retrieval rate±95% binomial CIs; g,h, Kruskal-Wallis test with Dunn’s correction; *p<0.05; **p<0.01.