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. Author manuscript; available in PMC: 2021 Nov 1.
Published in final edited form as: Metallomics. 2020 Oct 20;12(11):1791–1801. doi: 10.1039/d0mt00178c

Figure 5. Viable CFU and sensitivity to NO. stress do not change following NO.-challenge.

Figure 5.

(A) Viable CFU, shown as a percentage of initial CFU, remained consistent between wild-type (blue) and mntH sitA ΔzupT (orange) S. Typhimurium during the prolonged lag phase resulting from NO. stress. There was no significant difference in viable CFU between the two strains except at 2 hr and 5 hr, when the mutant displayed a small but statistically significant increased percentage of viable CFU (p=0.011 and p=0.013, respectively). Following recovery from the stress, normal growth kinetics resumed, and CFU began to increase with wild-type showing significantly greater viable CFU than mntH sitA ΔzupT from 11–14 hr (p=0.038, p=0.032, p=0.025, p=0.004). Statistical significance was determined by unpaired two-tailed t-test. (B &C) Wild-type and mntH sitA ΔzupT S. Typhimurium from NO. sensitivity assays that had been grown either in LB or LB with 5 mM SperNO were subcultured and used as the starting inocula for subsequent sensitivity assays. The population of mntH sitA ΔzupT cells arising from the initial NO. challenge (pale orange dashed lines) was not more resistant to NO. than mntH sitA ΔzupT cells that had not previously been exposed to NO. (dark orange dashed lines) in either LB (B) or M9 (C) medium.