Extended Data Fig. 6. IFIT2 enhances the translational efficiency of influenza virus NP mRNA.

a-c, Full dataset underlying results presented in Fig 4d. Polysome profiling was performed on WT and IFIT2^−/− A549 cells infected with WSN. a, The polysome profile is shown again for clarity. b, qRT-PCR quantification of β-actin or c, NP mRNA in each fraction. The sum of less efficiently translated mRNA present in “light” polysome fractions (5 or less ribosomes per message) or efficiently translated mRNA in “heavy” polysome fractions (>5 ribosomes per message) is shown under each trace. Loss of IFIT2 shifts NP mRNA towards the top of the gradient, indicating less efficient translation than in WT cells. d, IFIT2^−/− 293 cells or two clones of WT cells were transfected with an IFIT2 expression vector or an empty vector control prior to infection with WSN. Proteins were detected by western blot.