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. 2020 Nov 6;8:565826. doi: 10.3389/fcell.2020.565826

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Copyright © 2020 Lee, Park, Kim, Lee, Koh and Kim.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Lumican stimulates preosteoblast viability and differentiation. (A) The viability and (B) directional migration of preosteoblast MC3T3-E1 cells were assessed by CCK-8 assay and a Boyden chamber system, respectively, after exposure to the indicated concentrations of lumican for 24 h. (C) Alkaline phosphatase (ALP) activity and (D) osteocalcin secretion of MC3T3-E1 cells in osteogenic medium containing 50 μg/mL ascorbic acid and 10 mM β-glycerophosphate with or without 10 nM lumican for 7 days. ALP activity was normalized by total cellular protein amounts. (E) Quantitative RT-PCR expression analysis of osteoblast differentiation markers in MC3T3-E1 cells cultured in osteogenic medium with or without 10 nM lumican for 3 or 7 days. (F) Luciferase activity of Runx2 after exposure to the indicated concentrations of lumican in osteogenic medium for 3 days. Data are presented as mean ± SEM. *P < 0.05 vs. control.