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. 2020 Oct 27;46(11):1090–1104. doi: 10.1007/s10886-020-01229-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Relative fold change in expression levels of three tomato defence related genes (LOX1, TPX1 and PR1) after MeSA application (MeSA), whitefly infestation (Tv) and MeSA plus whitefly infestation (MeTv). Data for each of the three genes are presented as average (n = 3) relative fold change compared with control transcript levels for each gene. Data analysis was performed on Dcq values and there were significant differences amongst treatments for all three genes following one-way ANOVA’s. The Tukey HSD post-hoc test was used to test differences between the treatments. There were significantly more LOX1 transcripts in the Tv and MeTv treatments, but significantly less transcripts in the MeSA treatment, compared with controls. There was also significantly more LOX1 transcripts in the Tv and MeTv treatments compared with the MeSA treatment. All three treatments had significantly higher amounts of TPX1 and PR1 transcripts compared with controls. There was also a significantly more PR1 transcripts in the MeTv treatment compared with the standalone MeSA treatment. Error bars display 95 % confidence intervals; this was constructed using Dcq values. Significant differences between treatments are annotated onto the graph, bars with different letters (a, b or c) denote a significant difference between treatments