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. 2020 Oct 29;46(11):1032–1046. doi: 10.1007/s10886-020-01227-w

Table 2.

Cuticular hydrocarbons identified from crude larval extracts of Tribolium confusum, T. castaneum, T. destructor and Oryzaephilus surinamensis

T. confusume T. castaneume T. destructore O. surinamensise
No.a Compoundb RIcalc RIlitd Mean ± SE (ng) (%) Mean ± SE (ng) (%) Mean ± SE (ng) (%) Mean ± SE (ng) (%)
1 n-C25 2498 2500   3.35 ± 0.39   4.40   2.15 ± 0.20  3.11 16.76 ± 2.11   24.04 0.07 ± 0.02  1.90
2 11-/13-MeC25 2533 2534   0.05 ± 0.00  0.07   0.08 ± 0.01  0.11
3 5-MeC25 2548 2550   0.36 ± 0.04  0.55
4 3-MeC25 2572 2571   0.04 ± 0.00   0.05   0.27 ± 0.03  0.38   0.43 ± 0.05  0.64
5 5,11-DiMeC25 2581 2577   0.32 ± 0.03  0.50
6 n-C26 2597 2600   0.67 ± 0.05   0.87   0.53 ± 0.03  0.78   1.89 ± 0.15  2.83 0.02 ± 0.00  0.65
7 10-/11-/12-MeC26 2634 2632   0.09 ± 0.01   0.11   0.10 ± 0.01  0.15
12-/13-MeC26   0.32 ± 0.03  0.48
8 4-MeC26 2656 2656   0.03 ± 0.00   0.03   0.13 ± 0.01  0.20
9 3-MeC26 2674 2673   0.13 ± 0.02  0.18   0.25 ± 0.03  0.37
10 n-C27 2700 2700 40.37 ± 4.36 49.55 19.13 ± 0.95   28.00 25.06 ± 1.53   38.63 0.16 ± 0.02  4.51
11 11-/13-MeC27 2731 2731   2.28 ± 0.18   2.98   4.32 ± 0.29  6.29   4.75 ± 0.33  7.30
12 5-MeC27 2748 2750   0.58 ± 0.06   0.72   0.80 ± 0.06  1.22
13 11,15-DiMeC27 2758 2762   0.35 ± 0.03  0.51
14 3-MeC27 2771 2773   1.16 ± 0.13   1.39   1.04 ± 0.07  1.52   1.52 ± 0.11  2.28
15 5, X-DiMeC27 2778 2781   0.35 ± 0.04   0.43   1.95 ± 0.13  3.01
16 n-C28 2797 2800   5.84 ± 0.68   7.04   3.24 ± 0.19  4.71   1.78 ± 0.11  2.87 0.13 ± 0.01  3.46
17 3,X-DiMeC28 2802 2807   2.15 ± 0.12  3.37
18 12-/13-/14-MeC28 2830 2831   0.21 ± 0.02   0.26   0.20 ± 0.02  0.29   0.33 ± 0.02  0.51
19 4-MeC28 2855 2856   0.09 ± 0.01   0.11   0.03 ± 0.00  0.05
20 3-MeC28 2874 2872   0.00 ± 0.00  0.00
21 4,14-DiMeC28 2886 2893   0.10 ± 0.01  0.16
22 n-C29 2899 2904 19.92 ± 2.96 22.71 30.36 ± 1.67   44.29   1.66 ± 0.08  2.73 0.45 ± 0.03   12.50
23 11-/13-MeC29 2928 2931   0.33 ± 0.03   0.41
11-/13-/15-MeC29   0.35 ± 0.03  0.51
11-/15-MeC29   0.18 ± 0.01  0.28
24 3-MeC29 2970 2978   0.22 ± 0.03   0.26   0.33 ± 0.02  0.47
25 n-C30 2995 3000   1.33 ± 0.13   2.01   1.51 ± 0.11  2.22   1.22 ± 0.11  2.05 0.48 ± 0.05   12.55
26 n-C31 3094 3100   2.02 ± 0.17   2.85   2.26 ± 0.19  3.29   1.50 ± 0.14  2.52 0.97 ± 0.08   26.04
27 n-C32 3194 3200   1.19 ± 0.15   1.85   1.23 ± 0.13  1.80   1.23 ± 0.12  2.08 0.60 ± 0.08   14.60
28 n-C33 3295 3300   1.46 ± 0.16   1.96   0.97 ± 0.10  1.40   0.76 ± 0.08  1.27 0.49 ± 0.08   11.66
29 n-C34 3395 3400 0.29 ± 0.06  6.52
30 n-C35 3495 3500 0.18 ± 0.04  3.95
31 n-C36 3595 3600 0.08 ± 0.02  1.66
Total 81.63 ± 8.53 99.99 68.52 ± 3.57 100.01 65.59 ± 3.60 100.00 3.93 ± 0.42 100.00

Mean amounts (ng ± SE larva− 1) and relative quantities (% in 1 larval equivalent, LE) are given

aPeak numbers refer to Fig. 1a, b

bFor the identification procedure see experimental part

cRIcal = Retention index calculated on a HP-5MS capillary column (30 m x 0.25 mm x 0.25 µm)

dRIlit = Retention index as reported for compounds analyzed on HP-5MS or similar columns in the database (http://www.pherobase.com/) and by Fürstenau and Hilker (2017) or others (peak 5 in Hebanowska et al. (1989, 1990) and Svensson et al. (2014); peak 9 in Gerhardt et al. (2016); peak 13 in Spiewok et al. (2006); peak No. 21 in Geiselhardt et al. (2009)). The provided literature RI values of dimethyl alkanes (entries 15 and 17) refer to unambiguously identified compounds described as 5,13-diMeC27 and 3,13-diMeC27

eFor the preparation of extracts see experimental part