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. 2020 Nov 19;10:20142. doi: 10.1038/s41598-020-77180-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Reverse western blot assays and cell migrations. Reverse western blot assays were performed using human angiogenesis antibody arrays (R&D systems), which detected the relative quantities of 55 cytokines in EGM-2 (a) and HLFCM (b). Representative images of the cytokine array membranes. Factors in red rectangles represent pro-angiogenic growth factors. Factors in blue rectangles represent anti-angiogenic growth factors. (c) The effect of pro-angiogenetic growth factors on HUVECs migration in collagen I coated Platypus migration plates was tested. The migration of HUVECs in EBM-2 (top left) and EGM-2 (bottom left) media as well as EGM-2 media supplemented with VEGF (top middle), PIGF (top right), HB-EGF (bottom middle), and IL-8 (bottom right) pro-angiogenetic growth factors were examined. (d) Fluorescence intensity for combinations test of the four pro-angiogenetic growth factors.