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. 2020 Nov 19;3:688. doi: 10.1038/s42003-020-01422-1

Fig. 4. C4 employs host autophagy pathway for its anti-plasmodial effect leading to parasite elimination from the host cells.

Fig. 4

a, b Effect of 10 µM C4 on P. berghei infection in control (WT HeLa) and autophagy-deficient (Atg5 knockdown in 4a; LC3B knockdown in 4b) HeLa cells. Here data is represented as box-plot (10–90 percentile), n = 3 independent experiments. Statistical significance was assessed using non-parametric two-tailed Mann-Whitney test. ns: non-significant, *P < 0.1, ***P < 0.001. The anti-parasitic effect of 1 µM C4 on P. berghei (c) and PfUIS3@Pb (d) infection in Huh7 cells can be reverted using the known autophagy inhibitor Chloroquine (50 µM). Here, C4 and Chloroquine were added to the cells 2 h before infection. The effect was analysed at 24 h post-infection using flow cytometry by quantifying the EEFs number in infected cells. Data is represented as box-plot (10–90 percentile). n = 3 (for 4c) and n = 4 (for 4d) independent experiments. Statistical significance was assessed using the unpaired two-tailed t-test. ***P < 0.001, ****P < 0.0001.