Fig. 6. C4 does not obstruct the intrinsic autophagy flux of the host cell.

a Representative immunoblot (n = 2 independent experiments) of p62 levels in HeLa cells that were kept in complete (control) and amino acid depleted medium (amino acid starvation) for 7 h. b The p62 levels before and after amino acid starvation were calculated as the ratio of p62 to γ tubulin (loading control). Data represents means ± SEM (n = 2 independent experiments). Statistical significance was assessed using the unpaired two-tailed t-test. ns: non-significant.