Table 1.
Results of mass spectrometry analyses of the purified Hel a 6 allergen.
| NCBI Acc. No. | Protein (organism) | Unique peptides in MALDI TOF/TOF | E value of protein | Unique peptides in LC ESI MS/MS | E value of peptide | Sequence coverage |
|---|---|---|---|---|---|---|
| GI: 1191633749 | Pectate lyase 2 (Helianthus annuus) | R.FGFFQVVNNNYDR.W | 0.00094 | R.FLAPDDAAK.K | 0.013 | 24% |
| R.ADAPESESMTWNWR.T | 0.26 | R.QAMADCAQGFAK.G | 6.9e−05 | |||
| R.WGTYAIGGSSAPTILSQGNR.F | 6e-007 | K.QIWIDHCSFSK.A | 0.0012 | |||
| K.VEITNGGLTLMDVK.N | 1.3e−06 | |||||
| K.VMLLGADDGHHQDK.N | 1.2e−05 | |||||
| R.WGTYAIGGSSAPTILSQGNR.F | 0.00022 |
Search parameters are 01 missed cleavage, p < 0.05 as significance threshold, 0.5 Da (precursor ions), and 1.2 Da (fragment ions) mass tolerance for MALDI TOF/TOF whereas 40 ppm (precursor ions) and 100 ppm (fragment ions) respectively for LC-ESI qTOF, cysteine carbamidomethylation as fixed modification, methionine oxidation as variable modification, + 1 peptide charge for MALDI TOF/TOF and + 2, + 3, + 4 for LC-ESI qTOF.