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Non-metric multidimensional scaling (nMDS) analysis representing bacterial compositions in the four metamorphosis life stages of C. transvaalensis. Stress = 0.13 (Bray–Curtis), n = 32 samples. Significant differences were found between the microbiota compositions of all four life stages (ANOSIM: R = 0.874, p < 0.001). Egg masses: yellow; larvae: green; pupae: blue; adults: red.
