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. 2020 Nov 19;10:20220. doi: 10.1038/s41598-020-77001-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Western blot analysis of in vitro neddylation. Conjugation of recombinant SUMO-PfNEDD8 (SN8) was carried out using parasite lysate and ATP at 37 °C for 1 h. The reactions were stopped and subjected to immunoblotting using anti-His antibody. The presence (+) or absence (−) of reaction component is indicated. The arrowhead indicates signal corresponding to unconjugated SN8. β-actin was used as a loading control for reactions containing the parasite lysate. The positions of proteins size markers are shown in kDa (M).