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. 2020 Nov 19;10:20220. doi: 10.1038/s41598-020-77001-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Western blot of parasite immunoprecipitates. The lysates of HA-PfNEDD8-expressing (HN8) and wild type (WT) parasites were immunoprecipitated with rabbit anti-HA antibodies. The eluates (Eluate) along with the extract (Input), flow-through (FT) and wash (Wash) were analyzed by western blotting using mouse anti-HA antibodies. The HN8 blot shows bands corresponding to free HA-PfNEDD8 (indicated with an asterisk) and high molecular weight conjugates (indicated with arrows). The arrowheads indicate non-specific signal in both the blots. The protein size markers are in kDa (M).