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. 2020 Nov 19;11:5785. doi: 10.1038/s41467-020-19558-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Time course depicting analysis time points during liver injury recovery following APAP overdose or PH. b Murine liver sections (5 μm, n = 3) show necrotic TUNEL-positive (brown, top) and proliferative PCNA-positive (brown, bottom) cells. Scale bar is 100 μm. c Bar graphs quantifying total TUNEL- and PCNA-positive area. Error bars are s.e.m., P < 0.05 (*), < 0.01 (**), and < 0.0001 (****) calculated using unpaired t test with Welch’s correction (two-tailed). d t-SNE plot of all high-quality hepatocytes (Methods) in the scRNA-Seq dataset. Cells are colored by injury mode and time point. SNN clusters outlined in black. e Heatmap of marker genes for all clusters outlined in (d). f, g Pericentral Hepatocyte Signature Score (PCH Signature Score) (left). Violin plot of normalized expression of Cyp2e1 (middle) and Glul (right); percent positive calculated as percentage of total cells in each condition above average normalized genes expression (dashed red line). Untreated (UT) and each post-treatment are plotted for APAP (f) and PH (g). Source data provided as a Source Data file.