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. 2020 Nov 19;3:687. doi: 10.1038/s42003-020-01407-0

Fig. 1. NSP4 is expressed in bone marrow-resident, granulocyte-macrophage progenitors (GMPs).

Fig. 1

Identification of the NSP4-expressing cell type. a Intracellular staining and flow cytometry analysis for NSP4 expression in bone marrow cells isolated from wild-type and Prss57−/− littermates. Density plots describe the gating strategy and histogram overlays of Prss57+/+, Prss57−/−, and rat IgG2b isotype staining are shown for each gated population: (1) LSK: Lin−, SCA-1+, CD117+ cells; (2) megakaryocyte/erythrocyte progenitors (MEP): Lin−, SCA-1−, CD117+, CD34−, CD16/32lo; (3) common myeloid progenitors (CMP): Lin−, SCA-1−, CD117+, CD34+, CD16/32lo; (4) granulocyte-macrophage progenitors (GMP): Lin−, SCA-1−, CD117+, CD34+, CD16/32hi. b Western blot for NSP4 in GMPs and CMPs. Data shown are representative of at least three independent experimental repeats. c Prss57 expression by RNA sequencing, shown as reads per kilobase per million (RPKM). Data are presented as mean ± s.d.; n = 4 biological replicates per genotype; ****P < 0.0001; two-way ANOVA and Bonferroni post hoc test. d Immunofluorescence staining for NSP4, myeloperoxidase (MPO), and nucleus (DAPI counterstain) in a total bone marrow smear; NSP4 and MPO colocalization is indicated by arrowheads; scale bar, 5 μm.