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. 2020 Nov 19;3:687. doi: 10.1038/s42003-020-01407-0

Fig. 4. NSP4 regulates histamine, serotonin, and heparin/heparan sulfate levels in GMPs.

Fig. 4

a NSP4 expression monitored by qPCR and Western blot during the differentiation of GMPs to mast cells in vitro. b Histamine and serotonin levels in “mast cell-primed” GMPs of Prss57+/+ and Prss57−/− littermates. Bars represent mean; n = 3 biological replicates per genotype; *P < 0.05, **P < 0.01; Student’s t test. c Disaccharide structure code (DSC) for the representation of all disaccharides derived from any glycosaminoglycan. Chemical structure of the sulfated disaccharides that are enriched in GMP and BMMC samples. d, e Total levels of heparin/heparan sulfate and its corresponding disaccharide profile in GMPs. Data are presented as mean (b, d) or mean ± s.d. (c, e); n = 3 or 4 biological replicates per genotype; *P < 0.01, **P < 0.001, ***P < 0.0001 for indicated comparisons; statistical significance was determined by the unpaired Student’s t test corrected for multiple comparisons using the Holm–Sidak method. Data shown are representative of at least two independent experimental repeats.