Fig. 5. NSP4 is required for immune complex-induced vascular leakage in the skin.

a Experimental setup for the passive cutaneous anaphylaxis (PCA) mouse model. b Vascular leakage in the skin of Prss57−/− mice and Prss57+/+ littermates based on extravasation of Evans blue dye in the IgE-mediated or IgG-mediated PCA model described in (a). Bars represent mean; n = 9–12 biological replicates per genotype; ***P < 0.001; Student’s t test. c Giemsa-stained cross-section of haired-skin (dorsum) isolated from Prss57−/− mice and wild-type littermates. The mast cells residing in the skin are connective-tissue mast cells, which are mostly located in the deep dermis. Scale bar, 100 μm; inset scale bar, 10 μm. d Mast cells were quantified in randomly selected 0.5 mm² regions of interest (ROIs) in the section of Giemsa-stained tissues obtained from Prss57+/+ and Prss57−/− mice. Bars represent mean; 16 randomly selected ROIs in 9 sections from 2 Prss57+/+ littermates, and 18 randomly selected ROIs in nine sections from two Prss57−/− mice were quantified; Student’s t test. Data shown are representative of at least two independent experimental repeats.