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. 2020 Nov 19;3:687. doi: 10.1038/s42003-020-01407-0

Fig. 7. Mast cells and histamine are required for NSP4-dependent, immune complex-induced vascular leakage.

Fig. 7

a Experimental setup for the adoptive transfer of neutrophils and mast cells in the K/BxN STA model. Neutrophils and BMMCs were isolated from Prss57+/+ and Prss57−/− littermates. b Representative images and c quantification of vascular leakage following neutrophil transfer and K/BxN serum. Data are presented as mean ± s.d.; n = 5 biological replicates per genotype; ****P < 0.0001; two-way ANOVA and Bonferroni post hoc test. d Representative images and e quantification of vascular leakage following mast cell transfer and K/BxN serum injection into Prss57+/+ and Prss57−/− littermates. Data are presented as mean ± s.d.; n = 3–5 biological replicates per genotype; ****P < 0.0001; two-way ANOVA and Bonferroni post hoc test. f Representative images and g quantification of vascular leakage following histamine injection. Data are presented as mean ± s.d.; n = 3 biological replicates per genotype; ****P < 0.0001; two-way ANOVA and Bonferroni post hoc test. Data shown are representative of at least two independent experimental repeats.