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. 2020 Nov 19;10:20165. doi: 10.1038/s41598-020-77159-y

Figure 1.

Figure 1

Shot knockdown triggers apoptosis in Drosophila wing discs. (A–D) Control wing discs or those expressing shotRNAi or sas-4RNAi were stained with phalloidin (actin; red) and cleaved caspase-3 (CC3; green) to mark apoptotic cells. Images were analyzed for the percent area of the wing pouch positive for CC3. *p < 0.01 compared to Control, ANOVA with Tukey’s post-hoc test. (E–H) Control wing discs or those expressing shotRNAi or sas-4RNAi were stained with phalloidin (actin; red) and TUNNEL (green) to mark apoptotic cells. Images were analyzed for the percent area of the wing pouch positive for CC3. *p < 0.01 compared to Control, ANOVA with Tukey’s post-hoc test. (I–L) Control wing discs or those expressing shotRNAi or sas-4RNAi were stained with phalloidin (actin; red) and active, phosphorylated JNK (pJNK; green). Images were analyzed for the percent area of the wing pouch positive for CC3. *p < 0.01 compared to Control, ANOVA with Tukey’s post-hoc test. (M–P) Control wing discs or those expressing shotRNAi or sas-4RNAi were stained with phalloidin (actin; red) and active, phosphorylated p38 (pp38; green) to mark apoptotic cells. Images were analyzed for the percent area of the wing pouch positive for CC3. *p < 0.01 compared to Control; ANOVA with Tukey’s post-hoc test.