Figure 6.

GADD45 and Ask1 are required for cell cycle arrest in Drosophila S2 cells following Shot knockdown. (A–C) Drosophila S2 cells stably expressing GFP:CID and mCherry: α-Tubulin were treated without (Control) or with RNAi targeting shot and gadd45. Cells were imaged from prior to nuclear envelope breakdown (NEBD) through anaphase onset. Images are representative of at least 10 cells. Timestamps are relative to NEBD. (D) Plots show timing from NEBD to anaphase onset for each recorded cell for the indicated treatment condition. Cells not progressing into anaphase after 3 h were considered to have undergone mitotic arrest (“Arrest”; indicated by percentages listed at top). *p < 0.01 compared to Control, ^#p < 0.01 compared to shot^RNAi; ANOVA with Tukey’s post-hoc test.