Table 4.
IC50 values and phenol content for the T. indica fractions ability to inhibit the activity of purified GSTs
| Extract of T. indica | IC50 value | Erythrocyte GST | Placenta GST | hGSTP1-1 |
|---|---|---|---|---|
| Dichloromethane | Concentration (μg/ml) | 64.54 ± 5.85 | 105.4 ± 3.3 | 93.74 ± 4.3 |
| Phenol (μg GAE/g DW) | 3.15 ± 0.28 (4.9%) | 5.14 ± 0.16 (4.9%) | 4.6 ± 0.2 (4.9%) | |
| Flavonoid (mg RE/g DW) | 2 ± 0.18 (3.1%) | 3.2 ± 0.1 (3%) | 2.9 ± 0.006 (3.1%) | |
| Ethyl acetate | Concentration (μg/ml) | 27 ± 1 | 56.66 ± 16.5 | 58 ± 15 |
| Phenol (μg GAE/g DW) | 3.3 ± 0.12 (12.2%) | 6.9 ± 2 (12.2%) | 7.08 ± 1.8 (12.2) | |
| Flavonoid (mg RE/g DW) | 1.3 ± 0.023 (4.8%) | 1.3 ± 0.37 (2.3%) | 1.33 ± 0.35 (2.3%) | |
| n-butanol | Concentration (μg/ ml) | 3 ± 0.7 | 6.6 ± 1.2 | 4.85 ± 0.35 |
| Phenol (μg GAE/g DW) | 1.13 ± 0.26 (37.6%) | 2.5 ± 0.45 (37.9%) | 1.83 ± 0.13 (37.7%) | |
| Flavonoid (mg RE/g DW) | 0.25 ± 0.05 (8.3%) | 0.55 ± 0.09 (8.3%) | 0.4 ± 0.03 (8.2) |
IC50: amount of extract which causes 50% inhibition of enzyme activity
μg GAE/g DW: μg of gallic acid equivalent per gram of extract dry weight
μg RE/g DW: μg of rutin equivalent per gram of extract dry weight
The values represent the mean ± SD of three independent experiments
The values in parentheses represent the percentage relative to concentration in μg/ml