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. 2020 Nov 20;6:74. doi: 10.1186/s40729-020-00270-2

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 9 — Immunocytochemical detection of activated platelets expressing a CD62P or b CD63. CaP-blasted cp-Ti and plain SUS316L plates were incubated with PRP for 60 min, washed, fixed, and subjected to immunocytochemical visualization. Green represents proteins reactive for a CD62P or b CD63, while red represents polymerized actin. c To show non-specific detection in extra-platelet spaces, PRP containing relatively low platelet counts was added on the plates and examined