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. 2020 Sep 7;79(12):1635–1643. doi: 10.1136/annrheumdis-2020-217904

Figure 5.

Figure 5

TRIM24 is an upstream regulator of receptor-interacting protein kinase-3 (RIP3) expression. (A and B) Reverse transcription PCR (RT-PCR) (left), quantitative PCR (qPCR) (right) and western blot (B) analysis of TRIM24, RIP3, matrix metalloproteinase 3 (MMP3) and COX2 in chondrocytes infected with Ad-C or Ad-Trim24 shRNA at the indicated multuplicity of infection (MOI) (n=5). (C and D) Knee joint cartilage of wild-type (WT) mice intra-articularly injected with Ad-C or Ad-Trim24 shRNA stained with Safranin-O (n=9). (C) Cartilage destruction, osteophyte formation and subchondral bone thickness were assessed by scoring. (D) TRIM24, RIP3, MMP3, matrix metalloproteinase 13 (MMP13) and COX2 immunostaining in cartilage injected with Ad-C or Ad-Trim24 shRNA. (E) Relative expression levels of the indicated proteins were determined from immunohistochemistry of cartilage every 2 weeks after destabilise the medial meniscus (DMM) surgery (n=9). Values are expressed as the mean and were analysed by one-way analysis of variance with Bonferroni’s test (A), a non-parametric Mann-Whitney U test (C; Osteoarthritis Research Society International (OARSI) grading, osteophyte formation), or a two-tailed t-test (C; subchondral bone plate thickness). Scale bar: 100 μm.