Figure 3.

Female and male mice have similar autophagic and apoptotic protein expression profiles. (a) Beclin1 protein expression [n = 8 (male and female); ns]. (b) Atg7 expression [n = 8 (male and female); ns]. (c) The expression ratio of Bax to Bcl-xL [n = 7 (male and female); ns]. (d) Representative blot images showing Atg7, Beclin1, Bcl-xL and Bax expression in the male and female mice. All proteins were analyzed in 2-month-old female and male wild-type animals by western blotting. The data were analyzed by two-tailed Student’s t-test. All results are expressed as the means ± S.E.M. Full-length blots are provided in Supplementary Fig. S3. Multiple t tests comparisons were corrected by controlling FDR. The summary with all statistical analyses are provided in Supplementary Material Table 1.