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. 2020 Nov 19;10:20166. doi: 10.1038/s41598-020-76754-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fused image: axial, coronal and sagittal views of the transmission ultrasound 3D speed of sound map fused with reflection image of mature human knee. This representation highlights the 3D nature of the reconstruction. The articular cartilage in the tibiofemoral space is clearly visible. The lateral and medial collateral ligaments are visible as (correct) high speed regions. The high speed muscle is also shown with the correct SOS being read off of the perfectly registered pure SOS (speed of sound) image.