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. 2020 Nov 19;10:20166. doi: 10.1038/s41598-020-76754-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Left panel: 3D rendering of the SOS reconstruction of the human knee. See supplemental movie file_1 for motion. Here and in movie the fat has been suppressed. This shows clearly, the advantage of the quantitative values that allow direct segmentation of the different tissue types.The articular cartilage is blue, the muscle is red, the sub-patellar tendon and other ligaments are pink. The bone is ivory. . Right panel: Alternative colorization of segmentation based on the speed of sound image provided by Lightstream Technologies (courtesy J. Helms). The fat is shown as yellow, the skin as pink, the muscle tissue is red, and the articular cartilage is blue. Ligament structures, such as the medial collateral ligament visible on the left is brown. The bone is gray and hollow. There is clear separation in the tibio-femoral space. See Supplemental Movie 2.