Figure 4.

Activation of GPR183 leads to cytokine production favoring Mtb control. Primary MN from healthy donors (n = 8) were infected for 2 h with Mtb H37Rv (MOI 10:1), 7a,25-OHC (100 nM), and/or GSK682753 (10 μM). Cells were washed and left with drugs for a further 22 h. Changes in the expression of (A) IFNB1, TNF, and IL10 were measured by qPCR and normalized to untreated infected cells. Concentrations of (B) IFN-b, TNF-a, and IL-10 in the culture supernatant were measured by ELISA. Data are presented as mean fold change ± SEM or min to max for box plots; *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001; paired t-test.