Figure 6.
GPR183KO mice have higher lung CFU, corresponding with increased expression of transcription factors regulating type I interferon responses. Mice were infected with 300 CFU of aerosol Mtb H37Rv. (A) Bacterial lung burden 2 weeks p.i. (B) Total histology lung score. RNA was isolated from Mtb-infected lung and blood samples 2 weeks p.i. (C) Gene expression of Ifnb1, Irf3, and Irf7 in the lungs, (D) Ifnb1, Irf3, and Irf7 in the blood, was measured by qRT-PCR using Hprt1 as reference gene. Data are presented as ± SEM; ns, P > 0.05; *P ≤ 0.05; **P ≤ 0.01.