Fig. 1.

Lumefantrine response phenotypes of parents and progeny from the 803 × GB4 cross. Parasites indicated in green carry the 803 pfmdr1 locus encoding N86 and Y184, whereas parasites indicated in yellow carry the GB4 pfmdr1 locus encoding 86Y and 184F. A Results from 72 h half-maximal effective concentration (EC₅₀) of growth inhibition assays for 22 progeny and parental lines using SYBR green, a sensitive fluorescence indicator of double-stranded DNA levels (Smilkstein et al., 2004). B Parasitemia of parental lines 803 and GB4 after a 24 h 500 nM LUM exposure with three biological replicates. C Days to 2% parasitemia recovery (DTR) after a 24 h 500 nM LUM exposure for 22 progeny and parental lines. D Scatter plot between the EC₅₀ and DTR values of the individual parents and progeny. 803 is indicated in green and GB4 is indicated in yellow. Data from individual phenotype determinations are provided in Table S1 and Table S2. EC₅₀ means and confidence intervals were calculated from three biological replicates for all parasites except 36H9 and 40E7 (n = 2). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

** Parental 803 and GB4 haplotypes are indicated by ‘1’ (green) and ‘2’ (yellow), respectively.

* UM DTR values are taken from Tables S1 and S2 of this report. DHA EC50s are from supplementary Table S1 of Sá et al. (2018), except for subsequently determined mean ± standard errors in the mean (SEM) values of 1.39±0.04 (37D9; n=37), 3.32±0.41 (36H9; n=3), 2.22±0.30 (43E5; n=3), 2.60±0.47 (44D4; n=3) published here. See the cited Tables for confidence intervals and SEMs. EC50 values are in nanomolar; DTR values in days. ND = not determined.