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. 2020 Nov 13;6(11):e05468. doi: 10.1016/j.heliyon.2020.e05468

Figure 3.

Figure 3

Effects of two explant orientations (adaxial or abaxial side in contact with the medium) and two leaf portions (basal or apical) on in vitro shoot organogenesis in ‘Delite’ rabbiteye blueberry using WPM culture medium supplemented with TDZ. Different views of explants and shoots under a stereomicroscope (a–d) and digital image capturing (e–f). (a) Adaxial x Basal, with many small (black arrow), medium (gray arrow), and large (white arrow) shoots. (b) Adaxial x Apical, with many small (black arrow), medium (gray arrow), and large (white arrow) shoots: (a, b) Those two adaxial treatments showed the best efficiency in regenerating shoots. (c) Abaxial x Basal with small (black arrow) and large (white arrow) shoots. (d) Abaxial x Apical, with small (black arrow) and medium (gray arrow) shoots. (e) Regenerated shoots after adventitious organogenesis. (f) In vitro regenerated shoots after adventitious organogenesis, subcultured into WPM culture medium supplemented with 2.5 μM zeatin, pictured ten weeks later. Abbreviations: TDZ, thidiazuron; WPM, woody plant medium.